De novo biosynthesis of β-arbutin in Corynebacterium glutamicum via pathway engineering and process optimization.

ABSTRACT:

Background

β-Arbutin, a hydroquinone glucoside found in pears, bearberry leaves, and various plants, exhibits antioxidant, anti-inflammatory, antimicrobial, and anticancer effects. β-Arbutin has wide applications in the pharmaceutical and cosmetic industries. However, the limited availability of high-performance strains limits the biobased production of β-arbutin.

Results

This study established the β-arbutin biosynthetic pathway in C. glutamicum ATCC13032 by introducing codon-optimized ubiC, MNX1, and AS. Additionally, the production titer of β-arbutin was increased by further inactivation of csm and trpE to impede the competitive metabolic pathway. Further modification of the upstream metabolic pathway and supplementation of UDP-glucose resulted in the final engineered stra

SUBMITTER: Zhang B 

PROVIDER: S-EPMC11197339 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature