ALKBH5 regulates chicken adipogenesis by mediating LCAT mRNA stability depending on m⁶A modification.

ABSTRACT:

Background

Previous studies have demonstrated the role of N6-methyladenosine (m⁶A) RNA methylation in various biological processes, our research is the first to elucidate its specific impact on LCAT mRNA stability and adipogenesis in poultry.

Results

The 6 100-day-old female chickens were categorized into high (n = 3) and low-fat chickens (n = 3) based on their abdominal fat ratios, and their abdominal fat tissues were processed for MeRIP-seq and RNA-seq. An integrated analysis of MeRIP-seq and RNA-seq omics data revealed 16 differentially expressed genes associated with to differential m⁶A modifications. Among them, ELOVL fatty acid elongase 2 (ELOVL2), pyruvate dehydrogenase kinase 4 (PDK4), fatty acid binding protein 9 (PMP2), fatty acid binding prot

SUBMITTER: Chao X 

PROVIDER: S-EPMC11197345 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature