Biosynthesis of β-nicotinamide mononucleotide from glucose via a new pathway in Bacillus subtilis.

ABSTRACT:

Introduction

β-nicotinamide mononucleotide (β-NMN) is an essential precursor of nicotinamide adenine dinucleotide (NAD⁺) and plays a key role in supplying NAD⁺ and maintaining its levels. Existing methods for NMN production have some limitations, including low substrate availability, complex synthetic routes, and low synthetic efficiency, which result in low titers and high costs.

Methods

We constructed high-titer, genetically engineered strains that produce NMN through a new pathway. Bacillus subtilis WB600 was used as a safe chassis strain. Multiple strains overexpressing NadE, PncB, and PnuC in various combinations were constructed, and NMN titers of different strains were compared via shake-flask culture.

Results

T

SUBMITTER: Tan Z 

PROVIDER: S-EPMC11197623 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature