Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors.

ABSTRACT: Post-translational modification (PTM) of antigens provides an additional source of specificities targeted by immune responses to tumors or pathogens, but identifying antigen PTMs and assessing their role in shaping the immunopeptidome is challenging. Here we describe the Protein Modification Integrated Search Engine (PROMISE), an antigen discovery pipeline that enables the analysis of 29 different PTM combinations from multiple clinical cohorts and cell lines. We expanded the antigen landscape, uncovering human leukocyte antigen class I binding motifs defined by specific PTMs with haplotype-specific binding preferences and revealing disease-specific modified targets, including thousands of new cancer-specific antigens that can be shared between patients and across cancer types. Furthermore

SUBMITTER: Kacen A 

PROVIDER: S-EPMC11197725 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature