TRPC5 is essential in endothelium-dependent contraction of aorta from diet-induced obese mice.

ABSTRACT: The role of the Ca²⁺-permeable ion channel TRPC5 in regulating vasocontraction in obesity is poorly understood. Here, we investigated whether TRPC5 contributes to vascular dysfunction in obesity by promoting endothelium-dependent contraction via activation of cytosolic phospholipase A2 (cPLA₂) in the aortic endothelial cells of obese mice. Acetylcholine-induced endothelium-dependent relaxation and contraction in the aorta were measured using wire myography. PLA₂ activity was measured by the fluorogenic PLA₂ substrate Bis-BODIPY™ FL C₁₁-PC. The intracellular Ca²⁺ level in response to acetylcholine was measured by Fluo-4 fluorescence. Endothelium-derived contracting factors were assessed by enzyme immunoassay. Diet-indu

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC11197789 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature