Profiling several key milk miRNAs and analysing their signalling pathways in dairy sheep breeds during peak and late lactation.

ABSTRACT:

Background

The comprehensive understanding of microRNAs (miRNAs) in sheep milk during various lactation periods and their impact on milk yield and composition remains limited.

Objectives

This study aimed to investigate the expression patterns of four highly expressed miRNAs in sheep milk and their association with milk composition and yield parameters during peak and late lactation stages.

Methods

A total of 40 healthy 4-year-old Akkaraman (n = 20) and Awassi (n = 20) ewes registered with the Ministry of Agriculture and Forestry of the Republic of Türkiye were used in the present study. For miRNA isolation from milk, the Qiagen miRNeasy Serum/Plasma Advanced Kit was utilised following the manufacturer's instructions. The expression levels of miRNAs were assessed using

SUBMITTER: Duman E 

PROVIDER: S-EPMC11198020 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature