Enhancement of transcription efficiency by TAR-Tat system increases the functional expression of human olfactory receptors.

ABSTRACT: Humans have approximately 400 different olfactory receptors (hORs) and recognize odorants through the repertoire of hOR responses. Although the cell surface expression of hORs is critical to evaluate their response, hORs are poorly expressed on the surface of heterologous cells. To address this problem, previous studies have focused on hOR transportation to the membrane. Nevertheless, the response pattern of hORs to odorants has yet to be successfully linked, and the response sensitivity still remains to be improved. In this study, we demonstrate that increasing the transcriptional level can result in a significant increase in cell surface and functional expression of hORs. We used the TAR-Tat system, which increases the transcription efficiency through positive feedback, and found that OR

SUBMITTER: Ojiro I 

PROVIDER: S-EPMC11198769 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature