Fitness landscape of substrate-adaptive mutations in evolved amino acid-polyamine-organocation transporters.

ABSTRACT: The emergence of new protein functions is crucial for the evolution of organisms. This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire new nutrients from a changing environment requires evolvability of transport functions. Here, we demonstrate the importance of environmental pressure in obtaining a new activity or altering a promiscuous activity in members of the amino acid-polyamine-organocation (APC)-type yeast amino acid transporters family. We identify APC members that have broader substrate spectra than previously described. Using in vivo experimental evolution, we evolve two of these transporter genes, AGP1 and PUT4, toward new substrate specificities. Single mutation

SUBMITTER: Karapanagioti F 

PROVIDER: S-EPMC11198987 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature