Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging.

ABSTRACT: Photothermal microscopy is a highly sensitive pump-probe method for mapping nanostructures and molecules through the detection of local thermal gradients. While visible photothermal microscopy and mid-infrared photothermal microscopy techniques have been developed, they possess inherent limitations. These techniques either lack chemical specificity or encounter significant light attenuation caused by water absorption. Here, we present an overtone photothermal (OPT) microscopy technique that offers high chemical specificity, detection sensitivity, and spatial resolution by employing a visible probe for local heat detection in the C-H overtone region. We demonstrate its capability for high-fidelity chemical imaging of polymer nanostructures, depth-resolved intracellular chemical mapping of c

SUBMITTER: Wang L 

PROVIDER: S-EPMC11199576 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature