Genomic barcoding for clonal diversity monitoring and control in cell-based complex antibody production.

ABSTRACT: Engineered mammalian cells are key for biotechnology by enabling broad applications ranging from in vitro model systems to therapeutic biofactories. Engineered cell lines exist as a population containing sub-lineages of cell clones that exhibit substantial genetic and phenotypic heterogeneity. There is still a limited understanding of the source of this inter-clonal heterogeneity as well as its implications for biotechnological applications. Here, we developed a genomic barcoding strategy for a targeted integration (TI)-based CHO antibody producer cell line development process. This technology provided novel insights about clone diversity during stable cell line selection on pool level, enabled an imaging-independent monoclonality assessment after single cell cloning, and eventually improv

SUBMITTER: Bauer N 

PROVIDER: S-EPMC11199663 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature