Human cerebral organoids: cellular composition and subcellular morphological features.

ABSTRACT:

Introduction

Human cerebral organoids (hCOs) derived from pluripotent stem cells are very promising for the study of neurodevelopment and the investigation of the healthy or diseased brain. To help establish hCOs as a powerful research model, it is essential to perform the morphological characterization of their cellular components in depth.

Methods

In this study, we analyzed the cell types consisting of hCOs after culturing for 45 days using immunofluorescence and reverse transcriptase qualitative polymerase chain reaction (RT-qPCR) assays. We also analyzed their subcellular morphological characteristics by transmission electron microscopy (TEM).

Results

Our results show the development of proliferative zones to be remarkably similar to those found in human brain dev

SUBMITTER: Mateos-Martinez P 

PROVIDER: S-EPMC11199856 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature