An intronic SNP affects skeletal muscle development by regulating the expression of TP63.

ABSTRACT:

Background

Porcine skeletal muscle development is pivotal for improving meat production. TP63, a transcription factor, regulates vital cellular processes, yet its role in skeletal muscle proliferation is unclear.

Methods

The effects of TP63 on skeletal muscle cell viability and proliferation were investigated using both mouse and porcine skeletal muscle myoblasts. Selective sweep analysis in Western pigs identified TP63 as a potential candidate gene for skeletal muscle development. The correlation between TP63 overexpression and cell proliferation was assessed using quantitative real-time PCR (RT-qPCR) and 5-ethynyl-2'-deoxyuridine (EDU).

Results

The study revealed a positive correlation between TP63 overexpression and skeletal muscle cell

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11199888 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature