A novel thin plate spline methodology to model tissue surfaces and quantify tumor cell invasion in organ-on-chip models.

ABSTRACT: Organ-on-chip (OOC) models can be useful tools for cancer drug discovery. Advances in OOC technology have led to the development of more complex assays, yet analysis of these systems does not always account for these advancements, resulting in technical challenges. A challenging task in the analysis of these two-channel microfluidic models is to define the boundary between the channels so objects moving within and between channels can be quantified. We propose a novel imaging-based application of a thin plate spline method - a generalized cubic spline that can be used to model coordinate transformations - to model a tissue boundary and define compartments for quantification of invaded objects, representing the early steps in cancer metastasis. To evaluate its performance, we applied our an

SUBMITTER: Elton E 

PROVIDER: S-EPMC11199902 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature