Programmable Enzymatic Reaction Network in Artificial Cell-Like Polymersomes.

ABSTRACT: The ability to precisely control in vitro enzymatic reactions in synthetic cells plays a crucial role in the bottom-up design of artificial cell models that can recapitulate the key cellular features and functions such as metabolism. However, integration of enzymatic reactions has been limited to bulk or microfluidic emulsions without a membrane, lacking the ability to design more sophisticated higher-order artificial cell communities for reconstituting spatiotemporal biological information at multiple length scales. Herein, droplet microfluidics is utilized to synthesize artificial cell-like polymersomes with distinct molecular permeability for spatiotemporal control of enzymatic reactions driven by external signals and fuels. The presence of a competing reverse enzymatic reaction that de

SUBMITTER: Seo H 

PROVIDER: S-EPMC11200095 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature