Rapid, Point-of-Care Microwave Lysis and Electrochemical Detection of <i>Clostridioides difficile</i> Directly from Stool Samples.

ABSTRACT: The rapid detection of the spore form of Clostridioides difficile has remained a challenge for clinicians. To address this, we have developed a novel, precise, microwave-enhanced approach for near-spontaneous release of DNA from C. difficile spores via a bespoke microwave lysis platform. C. difficile spores were microwave-irradiated for 5 s in a pulsed microwave electric field at 2.45 GHz to lyse the spore and bacteria in each sample, which was then added to a screen-printed electrode and electrochemical DNA biosensor assay system to identify presence of the pathogen's two toxin genes. The microwave lysis method released both single-stranded and double-stranded genome DNA from the bacterium at quantifiable concentrations between 0.02 μg/mL to 250 μg/mL allowing for sub

SUBMITTER: Joshi LT 

PROVIDER: S-EPMC11200505 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature