Stable long-term germline transmission of GFP transgenic rat via PiggyBac transposon mediated gene transfer.

ABSTRACT: Transgene silencing provides a significant challenge in animal model production via gene engineering using viral vectors or transposons. Selecting an appropriate strategy, contingent upon the species is crucial to circumvent transgene silencing, necessitating long-term observation of in vivo gene expression. This study employed the PiggyBac transposon to create a GFP rat model to address transgene silencing in rats. Surprisingly, transgene silencing occurred while using the CAG promoter, contrary to conventional understanding, whereas the Ef1α promoter prevented silencing. GFP expression remained stable through over five generations, confirming efficacy of the Ef1α promoter for long-term protein expression in rats. Additionally, GFP expression was consistently maintained at the cellular le

SUBMITTER: Jeon BJ 

PROVIDER: S-EPMC11201299 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature