PHE1-based IgG-like antibody platform provides a novel strategy for enhanced T-cell immunotherapy.

ABSTRACT:

Introduction

Bispecific antibodies (BsAbs) can simultaneously target two epitopes of different antigenic targets, bringing possibilities for diversity in antibody drug design and are promising tools for the treatment of cancers and other diseases. T-cell engaging bsAb is an important application of the bispecific antibody, which could promote T cell-mediated tumor cell killing by targeting tumor-associated antigen (TAA) and CD3 at the same time.

Methods

This study comprised antibodies purification, Elisa assay for antigen binding, cytotoxicity assays, T cell activation by flow cytometry in vitro and xenogenic tumor model in vivo.

Results

We present a novel bsAb platform named PHE-Ig technique to promote cognate heavy chain (HC)-light chain (LC) pairing b

SUBMITTER: Wang L 

PROVIDER: S-EPMC11201533 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature