Quantitative risk assessment model of the presence of porcine epidemic diarrhea and African swine fever viruses in spray-dried porcine plasma.

ABSTRACT:

Introduction

There are no microbiological regulatory limits for viruses in animal feed and feed ingredients.

Methods

A performance objective (PO) was proposed in this study to manufacture a spray-dried porcine plasma (SDPP) batch absent of any infectious viral particles. The PO levels of -7.0, -7.2, and -7.3 log TCID₅₀/g in SDPP were estimated for three batch sizes (10, 15, and 20 tons).

Results and discussion

A baseline survey on the presence of porcine epidemic diarrhea virus (PEDV) in raw porcine plasma revealed a concentration of -1.0 ± 0.6 log TCID₅₀/mL as calculated using a TCID₅₀-qPCR derived standard curve. The mean African swine fever virus (ASFV) concentration in raw plasma was estimated to be 0.6 log HAD₅₀/mL (0.1

SUBMITTER: Sampedro F 

PROVIDER: S-EPMC11202310 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature