Selection of Reference Genes for Expression Normalization by RT-qPCR in Dracocephalum moldavica L.

ABSTRACT: Dracocephalum moldavica is widely used as an ornamental, medicine, and perfume in industry. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) is widely and accurately utilized for gene expression evaluations. Selecting optimal reference genes is essential for normalizing RT-qPCR results. However, the identification of suitable reference genes in D. moldavica has not been documented. A total of 12 reference genes in D. moldavica were identified by PEG6000 (15%) treatment under hypertonia conditions in different tissues (roots, stem, leaves, flower, seeds and sepal) and during three stages of flower development, then used to validate the expression stability. There were four algorithms (delta Ct, geNorm, NormFinder, and BestKeeper) use

SUBMITTER: Li S 

PROVIDER: S-EPMC11202811 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature