The Development of an Isotope Dilution Mass Spectrometry Method for Interleukin-6 Quantification.

ABSTRACT: Inflammatory responses and tumor developments are closely related, with interleukin-6 (IL-6) playing important roles in both processes. IL-6 has been extensively identified as a potential tumor biomarker. This study developed an isotope dilution mass spectrometry (IDMS) method for quantifying IL-6 based on signature peptides. These peptides were screened by excluding those with missed cleavage or post-translational modification. The method's accuracy was verified using amino acid-based IDMS, in which purified IL-6 protein samples were quantified after hydrolyzing them into amino acids, and no significant difference was observed (p-value < 0.05). The method demonstrated good linearity and sensitivity upon testing. The specificity and matrix effect of the method were verified, and a p

SUBMITTER: Yu Z 

PROVIDER: S-EPMC11203838 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature