The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability.

ABSTRACT: As a low-calorie sugar, D-allulose is produced from D-fructose catalyzed by D-allulose 3-epimerase (DAE). Here, to improve the catalytic activity, stability, and processability of DAE, we reported a novel method by forming organic-inorganic hybrid nanoflowers (NF-DAEs) and co-immobilizing them on resins to form composites (Re-NF-DAEs). NF-DAEs were prepared by combining DAE with metal ions (Co²⁺, Cu²⁺, Zn²⁺, Ca²⁺, Ni²⁺, Fe²⁺, and Fe³⁺) in PBS buffer, and were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction. All of the NF-DAEs showed higher catalytic activities than free DAE, and the NF-DAE with Ni²⁺ (NF-DAE-Ni) reached the highest relative a

SUBMITTER: Ding W 

PROVIDER: S-EPMC11203923 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature