Simple Determination of Affinity Constants of Antibodies by Competitive Immunoassays.

ABSTRACT: The affinity constant, also known as the equilibrium constant, binding constant, equilibrium association constant, or the reciprocal value, the equilibrium dissociation constant (K_d), can be considered as one of the most important characteristics for any antibody-antigen pair. Many methods based on different technologies have been proposed and used to determine this value. However, since a very large number of publications and commercial datasheets do not include this information, significant obstacles in performing such measurements seem to exist. In other cases where such data are reported, the results have often proved to be unreliable. This situation may indicate that most of the technologies available today require a high level of expertise and effort that does not seem to

SUBMITTER: Fischer J 

PROVIDER: S-EPMC11206456 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature