Novel Betanucleorhabdoviruses Infecting Elderberry (<i>Sambucus nigra</i> L.): Genome Characterization and Genetic Variability.

ABSTRACT: The genus Betanucleorhabdovirus includes plant viruses with negative sense, non-segmented, single-stranded RNA genomes. Here, we characterized putative novel betanucleorhabdoviruses infecting a medically important plant, elderberry. Total RNA was purified from the leaves of several plants, ribodepleted and sequenced using the Illumina platform. Sequence data analysis led to the identification of thirteen contigs of approximately 13.5 kb, showing a genome structure (3'-N-P-P3-M-G-L-5') typical of plant rhabdoviruses. The detected isolates showed 69.4 to 98.9% pairwise nucleotide identity and had the highest identity among known viruses (64.7-65.9%) with tomato betanucleorhabdovirus 2. A detailed similarity analysis and a phylogenetic analysis allowed us to discriminate the elderberry

SUBMITTER: Safarova D 

PROVIDER: S-EPMC11206775 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature