Placental Methylglyoxal in Preeclampsia: Vascular and Biomarker Implications.

ABSTRACT:

Background

Preeclampsia is a multifaceted syndrome that includes maternal vascular dysfunction. We hypothesize that increased placental glycolysis and hypoxia in preeclampsia lead to increased levels of methylglyoxal (MGO), consequently causing vascular dysfunction.

Methods

Plasma samples and placentas were collected from uncomplicated and preeclampsia pregnancies. Uncomplicated placentas and trophoblast cells (BeWo) were exposed to hypoxia. The reactive dicarbonyl MGO and advanced glycation end products (N^ε-(carboxymethyl)lysine [CML], N^ε-(carboxyethyl)lysine [CEL], and MGO-derived hydroimidazolone [MG-H]) were quantified using liquid chromatography-tandem mass spectrometry. The activity of GLO1 (glyoxalase-1), that is, the enzyme detoxifying MGO, was

SUBMITTER: Vangrieken P 

PROVIDER: S-EPMC11208051 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature