The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus.

ABSTRACT: In bacteria, the availability of environmental inorganic phosphate is typically sensed by the conserved PhoR-PhoB two-component signal transduction pathway, which uses the flux through the PstSCAB phosphate transporter as a readout of the extracellular phosphate level to control phosphate-responsive genes. While the sensing of environmental phosphate is well-investigated, the regulatory effects of cytoplasmic phosphate are unclear. Here, we disentangle the physiological and transcriptional responses of Caulobacter crescentus to changes in the environmental and cytoplasmic phosphate levels by uncoupling phosphate uptake from the activity of the PstSCAB system, using an additional, heterologously produced phosphate transporter. This approach reveals a two-pronged response of C. crescentus to

SUBMITTER: Billini M 

PROVIDER: S-EPMC11208175 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature