Quantitative and dynamic profiling of human gut core microbiota by real-time PCR.

ABSTRACT: The human gut microbiota refers to a diverse community of microorganisms that symbiotically exist in the human intestinal system. Altered microbial communities have been linked to many human pathologies. However, there is a lack of rapid and efficient methods to assess gut microbiota signatures in practice. To address this, we established an appraisal system containing 45 quantitative real-time polymerase chain reaction (qPCR) assays targeting gut core microbes with high prevalence and/or abundance in the population. Through comparative genomic analysis, we selected novel species-specific genetic markers and primers for 31 of the 45 core microbes with no previously reported specific primers or whose primers needed improvement in specificity. We comprehensively evaluated the performance of

SUBMITTER: Yan Z 

PROVIDER: S-EPMC11208268 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature