Magnesium enhances aurintricarboxylic acid's inhibitory action on the plasma membrane Ca²⁺-ATPase.

ABSTRACT: Our research aimed to elucidate the mechanism by which aurintricarboxylic acid (ATA) inhibits plasma membrane Ca²⁺-ATPase (PMCA), a crucial enzyme responsible for calcium transport. Given the pivotal role of PMCA in cellular calcium homeostasis, understanding how it is inhibited by ATA holds significant implications for potentially regulating physiopathological cellular processes in which this pump is involved. Our experimental findings revealed that ATA employs multiple modes of action to inhibit PMCA activity, which are influenced by ATP but also by the presence of calcium and magnesium ions. Specifically, magnesium appears to enhance this inhibitory effect. Our experimental and in-silico results suggest that, unlike those reported in other proteins, ATA complexed with magnesi

SUBMITTER: Souto-Guevara CA 

PROVIDER: S-EPMC11208427 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature