Implications of mitochondrial membrane potential gradients on signaling and ATP production analyzed by correlative multi-parameter microscopy.

ABSTRACT: The complex architecture and biochemistry of the inner mitochondrial membrane generate ultra-structures with different phospholipid and protein compositions, shapes, characteristics, and functions. The crista junction (CJ) serves as an important barrier separating the cristae (CM) and inner boundary membranes (IBM). Thereby CJ regulates the movement of ions and ensures distinct electrical potentials across the cristae (ΔΨ_C) and inner boundary (ΔΨ_IBM) membranes. We have developed a robust and flexible approach to visualize the CJ permeability with super-resolution microscopy as a readout of local mitochondrial membrane potential (ΔΨ_mito) fluctuations. This method involves analyzing the distribution of TMRM fluorescence intensity in a model that is restricted

SUBMITTER: Gottschalk B 

PROVIDER: S-EPMC11208492 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature