Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes).

ABSTRACT: Japanese medaka (Oryzias latipes) has been used as a model organism in different research fields, including reproductive physiology. Sperm motility is the most important marker for male fertility in fish and, thus, reproduction success. However, because of small volume of ejaculate and short motility duration, it is still challenging to manage the sperm collection and analysis in small model fish. In the present study, we aimed to investigate sperm motility and to optimize sperm collection, short-term sperm storage, and cryopreservation in Japanese medaka (Oryzias latipes). Using two different approaches for sperm collection: testes dissection and abdominal massage, different housing conditions and activating the sperm with different activation solutions, we investigated immediate sperm mo

SUBMITTER: Sayyari A 

PROVIDER: S-EPMC11208518 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature