PROMER technology: A new real-time PCR tool enabling multiplex detection of point mutation with high specificity and sensitivity.

ABSTRACT: Real-time polymerase chain reaction (real-time PCR) is a powerful tool for the precise quantification of nucleic acids in various applications. In cancer management, the monitoring of circulating tumor DNA (ctDNA) from liquid biopsies can provide valuable information for precision care, including treatment selection and monitoring, prognosis, and early detection. However, the rare and heterogeneous nature of ctDNA has made its precise detection and quantification challenging, particularly for ctDNA containing hotspot mutations. We have developed a new real-time PCR tool, PROMER technology, which enables the precise and sensitive detection of ctDNA containing cancer-driven single-point mutations. The PROMER functions as both a PRObe and priMER, providing enhanced detection specificity. We v

SUBMITTER: Nam H 

PROVIDER: S-EPMC11208725 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature