Decreased LDHB expression in breast tumor cells causes NK cell activation and promotes tumor progression.

ABSTRACT:

Objective

Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B (LDHB) has been detected in breast cancer but the function of LDHB remains unknown.

Methods

Western blot was used to analyze LDHB expression in breast cancer cells. The impact of LDHB on tumor cell migration and invasion was determined using Transwell assays, wound healing assays, and a mouse lung metastasis model. Subcutaneous tumor formation, a natural killer (NK) cell cytotoxicity assay, and flow cytometry evaluated NK cell activation. Immunofluorescence and quantitative real-time PCR detected NK cell activation markers. Kaplan-Meier analysis evaluated the effect of immune cell infiltration on prognosis. Single-sample gene set

SUBMITTER: Luo Z 

PROVIDER: S-EPMC11208901 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature