Generation of monoclonal antibody against 6-Keto PGF_1α and development of ELISA for its quantification in culture medium.

ABSTRACT: Prostacyclin or prostaglandin I₂ (PGI₂), a metabolite of arachidonic cyclooxygenase pathway, has been demonstrated as an effector of adipocyte differentiation. However, due to its instability in biological fluid, it is difficult to evaluate the role of PGI₂ in regulating adipocyte differentiation in different stages in culture. Therefore, this study aimed to establish a simple and rapid method for the production of monoclonal antibody against 6-Keto PGF₁α, a stable PGI₂ metabolite, and its quantification to determine the role of PGI₂ in culture medium. Eight-week-old female BALB/c mice were immunized with the hapten of 6-Keto PGF₁α and BSA for several weeks until a higher antibody titer (absorbance value > 0.9 at

SUBMITTER: Chowdhury MMI 

PROVIDER: S-EPMC11208916 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature