Diagnosis and Characterization of Plant Viruses Using HTS to Support Virus Management and Tomato Breeding.

ABSTRACT: Viral diseases pose a significant threat to tomato crops (Solanum lycopersicum L.), one of the world's most economically important vegetable crops. The limited genetic diversity of cultivated tomatoes contributes to their high susceptibility to viral infections. To address this challenge, tomato breeding programs must harness the genetic resources found in native populations and wild relatives. Breeding efforts may aim to develop broad-spectrum resistance against the virome. To identify the viruses naturally infecting 19 advanced lines, derived from native tomatoes, high-throughput sequencing (HTS) of small RNAs and confirmation with PCR and RT-PCR were used. Single and mixed infections with tomato mosaic virus (ToMV), tomato golden mosaic virus (ToGMoV), and pepper huasteco yellow

SUBMITTER: Gonzalez-Perez E 

PROVIDER: S-EPMC11209215 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature