Applying Flow Virometry to Study the HIV Envelope Glycoprotein and Differences Across HIV Model Systems.

ABSTRACT: The HIV envelope glycoprotein (Env) is a trimeric protein that facilitates viral binding and fusion with target cells. As the sole viral protein on the HIV surface, Env is important both for immune responses to HIV and in vaccine designs. Targeting Env in clinical applications is challenging due to its heavy glycosylation, high genetic variability, conformational camouflage, and its low abundance on virions. Thus, there is a critical need to better understand this protein. Flow virometry (FV) is a useful methodology for phenotyping the virion surface in a high-throughput, single virion manner. To demonstrate the utility of FV to characterize Env, we stained HIV virions with a panel of 85 monoclonal antibodies targeting different regions of Env. A broad range of antibodies yielded robust st

SUBMITTER: Burnie J 

PROVIDER: S-EPMC11209363 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature