A Coiled-Coil Nucleotide-Binding Domain Leucine-Rich Repeat Receptor Gene <i>MeRPPL1</i> Plays a Role in the Replication of a Geminivirus in Cassava.

ABSTRACT: Disease resistance gene (R gene)-encoded nucleotide-binding leucine-rich repeat proteins (NLRs) are critical players in plant host defence mechanisms because of their role as receptors that recognise pathogen effectors and trigger plant effector-triggered immunity (ETI). This study aimed to determine the putative role of a cassava coiled-coil (CC)-NLR (CNL) gene MeRPPL1 (Manes.12G091600) (single allele) located on chromosome 12 in the tolerance or susceptibility to South African cassava mosaic virus (SACMV), one of the causal agents of cassava mosaic disease (CMD). A transient protoplast system was used to knock down the expression of MeRPPL1 by clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 (CRISPR-Cas9). The MeRPPL1-targe

SUBMITTER: Ramulifho E 

PROVIDER: S-EPMC11209366 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature