MiR-122-5p Promotes Cowshed Particulate Matter2.5-Induced Apoptosis in NR8383 by Targeting COL4A1.

ABSTRACT: It is well known that Particulate Matter2.5 (PM_2.5) has a major adverse effect on the organism. However, the health hazards of livestock farm PM_2.5 to humans and animals are not yet known, and the role of miRNAs in the cellular damage induced by livestock farm PM_2.5 is also unclear. Therefore, our study used cowshed PM_2.5 to stimulate rat alveolar macrophage NR8383 to construct an in vitro injury model to investigate the effect of miR-122-5p on PM_2.5-induced apoptosis in the NR8383. The level of apoptosis was quantified by flow cytometry and Hoechst 33342/PI double staining. Furthermore, the potential target gene Collagen type IV alpha (COL4A1) of miR-122-5p was identified through the use of bioinformatics methods. The results d

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11209608 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature