A New Approach for On-Chip Production of Biological Microgels Using Photochemical Cross-Linking.

ABSTRACT: Photochemical cross-linking is a key step for manufacturing microgels in numerous applications, including drug delivery, tissue engineering, material production, and wound healing. Existing photochemical cross-linking techniques in microfluidic devices rely on UV curing, which can cause cell and DNA damage. We address this challenge by developing a microfluidic workflow for producing microgels using visible light-driven photochemical cross-linking of aqueous droplets dispersed in a continuous oil phase. We report a proof-of-concept to construct microgels from the protein Bovine Serum Albumin (BSA) with [Ru(bpy)₃]²⁺ mediated cross-linking. By controlling the capillary number of the continuous and dispersed phases, the volumetric flow rate, and the photochemical reactio

SUBMITTER: Del Giudice F 

PROVIDER: S-EPMC11209654 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature