Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma.

ABSTRACT: Background: Immunotherapy has demonstrated its potential to improve the prognosis of patients with hepatocellular carcinoma (HCC); however, patients' responses to immunotherapy vary a lot. A comparative analysis of the tumor microenvironment (TME) in responders and non-responders is expected to unveil the mechanisms responsible for the immunotherapy resistance and provide potential treatment targets. Methods: We performed sequencing analyses using 10x Genomics technology on six HCC patients who responded to anti-PD-1 therapy and one HCC patient who did not respond. Additionally, we obtained single cell data from untreated, responsive, and nonresponsive HCC patients from public databases, and used part of the datasets as a validation cohort. These data were integrated using al

SUBMITTER: Li Y 

PROVIDER: S-EPMC11209711 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature