Engineering strategies for enhanced 1', 4'-trans-ABA diol production by Botrytis cinerea.

ABSTRACT:

Background

Currently, industrial fermentation of Botrytis cinerea is a significant source of abscisic acid (ABA). The crucial role of ABA in plants and its wide range of applications in agricultural production have resulted in the constant discovery of new derivatives and analogues. While modifying the ABA synthesis pathway of existing strains to produce ABA derivatives is a viable option, it is hindered by the limited synthesis capacity of these strains, which hinders further development and application.

Results

In this study, we knocked out the bcaba4 gene of B. cinerea TB-31 to obtain the 1',4'-trans-ABA-diol producing strain ZX2. We then studied the fermentation broth of the batch-fed fermentation of the ZX2 strain using metabolomic analysis. The results showed significa

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11210036 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature