TRIM21 and Fc-engineered antibodies: decoding its complex antibody binding mode with implications for viral neutralization.

ABSTRACT: TRIM21 is a pivotal effector in the immune system, orchestrating antibody-mediated responses and modulating immune signaling. In this comprehensive study, we focus on the interaction of TRIM21 with Fc engineered antibodies and subsequent implications for viral neutralization. Through a series of analytical techniques, including biosensor assays, mass photometry, and electron microscopy, along with structure predictions, we unravel the intricate mechanisms governing the interplay between TRIM21 and antibodies. Our investigations reveal that the TRIM21 capacity to recognize, bind, and facilitate the proteasomal degradation of antibody-coated viruses is critically dependent on the affinity and avidity interplay of its interactions with antibody Fc regions. We suggest a novel binding mechanism

SUBMITTER: Reusch J 

PROVIDER: S-EPMC11210195 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature