Disordered C-terminal domain drives spatiotemporal confinement of RNAPII to enhance search for chromatin targets.

ABSTRACT: Efficient gene expression requires RNA polymerase II (RNAPII) to find chromatin targets precisely in space and time. How RNAPII manages this complex diffusive search in three-dimensional nuclear space remains largely unknown. The disordered carboxy-terminal domain (CTD) of RNAPII, which is essential for recruiting transcription-associated proteins, forms phase-separated droplets in vitro, hinting at a potential role in modulating RNAPII dynamics. In the present study, we use single-molecule tracking and spatiotemporal mapping in living yeast to show that the CTD is required for confining RNAPII diffusion within a subnuclear region enriched for active genes, but without apparent phase separation into condensates. Both Mediator and global chromatin organization are required for sustaining RN

SUBMITTER: Ling YH 

PROVIDER: S-EPMC11210292 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature