Sivelestat improves acute lung injury by inhibiting PI3K/AKT/mTOR signaling pathway.

ABSTRACT:

Objective

To investigate the therapeutic effect and mechanism of sivelestat sodium on acute lung injury (AIL).

Methods

A rat model for ALI/acute respiratory distress syndrome (ALI/ARDS) was established. Pathological examination of lung tissue was conducted to assess lung injury. Blood gas in the arteries was measured using a blood analyzer. Changes in PaO2, PaO2/FiO2, and lung wet/dry (W/D) weight ratio were carefully compared. ELISA assay was conducted to estimate cell adhesion and inflammation response. Finally, real-time reverse transcription polymerase chain reaction and western blotting assay was used to determine the activation of PI3K/AKT/mTOR pathway.

Results

ARDS in vivo model was successfully constructed by LPS injection. Compared with the sham group, PaO2 a

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC11210789 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature