Precise measurement of molecular phenotypes with barcode-based CRISPRi systems.

ABSTRACT: Genome-wide CRISPR-Cas9 screens have untangled regulatory networks and revealed the genetic underpinnings of diverse biological processes. Their success relies on experimental designs that interrogate specific molecular phenotypes and distinguish key regulators from background effects. Here, we realize these goals with a generalizable platform for CRISPR interference with barcoded expression reporter sequencing (CiBER-seq) that dramatically improves the sensitivity and scope of genome-wide screens. We systematically address technical factors that distort phenotypic measurements by normalizing expression reporters against closely-matched control promoters, integrated together into the genome at single copy. To test our ability to capture post-transcriptional and post-translational regulatio

SUBMITTER: Lobel JH 

PROVIDER: S-EPMC11213135 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature