Assessing the effects of experimental bacterial challenge with Pasteurella multocida and ampicillin on the respiratory microbiota of pre-weaned Holstein calves.

ABSTRACT: The association between changes in the respiratory microbiota and Bovine Respiratory Disease (BRD) in dairy calves is not well understood. We investigated characteristics of the nasopharyngeal (NP) microbiota associated with BRD following Pasteurella multocida infection. We also evaluated the effect of ampicillin on the respiratory microbiota. Calves (n = 30) were inoculated with P. multocida and randomly allocated into an antibiotic group (AMP; n = 17) or placebo group (PLAC; n = 11) when lung lesions developed. Deep NP swabs (DNPS) were collected before and after challenge. Monitoring was performed daily until euthanasia at day 14. Swabs and tissue samples were collected for analysis. The V4 hypervariable region of the 16 S rRNA gene was amplified and sequenced on an Illumina MiSeq. Incr

SUBMITTER: Raabis SM 

PROVIDER: S-EPMC11215343 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature