Azithromycin resistance in Escherichia coli and Salmonella from food-producing animals and meat in Europe.

ABSTRACT:

Objectives

To characterize the genetic basis of azithromycin resistance in Escherichia coli and Salmonella collected within the EU harmonized antimicrobial resistance (AMR) surveillance programme in 2014-18 and the Danish AMR surveillance programme in 2016-19.

Methods

WGS data of 1007 E. coli [165 azithromycin resistant (MIC > 16 mg/L)] and 269 Salmonella [29 azithromycin resistant (MIC > 16 mg/L)] were screened for acquired macrolide resistance genes and mutations in rplDV, 23S rRNA and acrB genes using ResFinder v4.0, AMRFinder Plus and custom scripts. Genotype-phenotype concordance was determined for all isolates. Transferability of mef(C)-mph(G)-carrying plasmids was assessed by conjugation experiments.

Results

mph(A), mph(B), mef(B), erm(B) and mef(C)-mph(G) were

SUBMITTER: Ivanova M 

PROVIDER: S-EPMC11215539 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature