Drosophila melanogaster Set8 and L(3)mbt function in gene expression independently of histone H4 lysine 20 methylation.

ABSTRACT: Monomethylation of lysine 20 of histone H4 (H4K20me1) is catalyzed by Set8 and thought to play important roles in many aspects of genome function that are mediated by H4K20me binding proteins. We interrogated this model in a developing animal by comparing in parallel the transcriptomes of Set8 ^null , H4 ^K20R/A , and l(3)mbt mutant Drosophila melanogaster We found that the gene expression profiles of H4 ^K20A and H4 ^K20R larvae are markedly different than Set8 ^null larvae despite similar reductions in H4K20me1. Set8 ^null mutant cells have a severely disrupted transcriptome and fail to proliferate in vivo, but these phenotypes are

SUBMITTER: Crain AT 

PROVIDER: S-EPMC11216177 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature