Slow G-Quadruplex Conformation Rearrangement and Accessibility Change Induced by Potassium in Human Telomeric Single-Stranded DNA.

ABSTRACT: The guanine-rich telomeric repeats can form G-quadruplexes (G4s) that alter the accessibility of the single-stranded telomeric overhang. In this study, we investigated the effects of Na⁺ and K⁺ on G4 folding and accessibility through cation introduction and exchange. We combined differential scanning calorimetry (DSC), circular dichroism (CD), and single molecule Förster resonance energy transfer (smFRET) to monitor the stability, conformational dynamics, and complementary strand binding accessibility of G4 formed by single-stranded telomeric DNA. Our data showed that G4 formed through heating and slow cooling in K⁺ solution exhibited fewer conformational dynamics than G4 formed in Na⁺ solution, which is consistent with the higher thermal stabili

SUBMITTER: Lacen AN 

PROVIDER: S-EPMC11216195 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature