Glycan Isomer Identification Using Ultraviolet Photodissociation Initiated Radical Chemistry.

ABSTRACT: Glycans are fundamental biological macromolecules, yet despite their prevalence and recognized importance, a number of unique challenges hinder routine characterization. The multiplicity of OH groups in glycan monomers easily afford branched structures and alternate linkage sites, which can result in isomeric structures that differ by minute details. Herein, radical chemistry is employed in conjunction with mass spectrometry to enable rapid, accurate, and high throughput identification of a challenging series of closely related glycan isomers. The results are compared with analysis by collision-induced dissociation, higher-energy collisional dissociation, and ultraviolet photodissociation (UVPD) at 213 nm. In general, collision-based activation struggles to produce characteristic fragmenta

SUBMITTER: Riggs DL 

PROVIDER: S-EPMC11216535 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature