Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm.

ABSTRACT:

Purpose

This study aimed to identify the altered pathways and genes associated with freezing damage in human sperm during cryopreservation by multiomics analysis.

Materials and methods

Fifteen fresh human semen samples were collected for transcriptomic analysis, and another 5 fresh human semen samples were obtained for metabolomic analysis. For each semen sample, 1 mL was cryopreserved, and another 1 mL was left untreated for paired design. The results were then combined with previously published proteomic results to identify key genes/pathways.

Results

Cryopreservation significantly reduced sperm motility and mitochondrial structure. Transcriptomic analysis revealed altered mitochondrial function, including changes in tRNA-methyltransferase activity and adenosine tri

SUBMITTER: Fu L 

PROVIDER: S-EPMC11216965 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature