Naturally sterile Mus spretus hybrids are suitable for the generation of pseudopregnant embryo transfer recipients.

ABSTRACT: For the preparation of embryo transfer recipients, surgically vasectomized mice are commonly used, generated by procedures associated with pain and discomfort. Sterile transgenic strains provide a nonsurgical replacement, but their maintenance requires breeding and genotyping procedures. We have previously reported the use of naturally sterile STUSB6F1 hybrids for the production of embryo transfer recipients and found the behavior of these recipients to be indistinguishable from those generated by vasectomized males. The method provides two substantial 3R impacts: refinement (when compared with surgical vasectomy) and reduction in breeding procedures (compared with sterile transgenic lines). Despite initial promise, the 3Rs impact of this innovation was limited by difficulties in breeding

SUBMITTER: Preece C 

PROVIDER: S-EPMC11216974 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature